Complete Genome Sequence of the Industrial Strain Bacillus megaterium WSH-002
نویسندگان
چکیده
منابع مشابه
Complete genome sequence of the industrial strain Bacillus megaterium WSH-002.
Bacillus megaterium, an industrial strain, has been widely used in protein production and the vitamin C industry. Here we reported a finished, annotated, and compared 4.14-Mbp high-quality genome sequence of B. megaterium WSH-002, which is the companion strain for Ketogulonicigenium vulgare in the vitamin C industry and is stocked in our laboratory.
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Alanine dehydrogenase (L-AlaDH) from Bacillus megaterium WSH-002 catalyses the NAD⁺-dependent interconversion of L-alanine and pyruvate. The enzyme was expressed in Escherichia coli BL21 (DE3) cells and purified with a His6 tag by Ni²⁺-chelating affinity chromatography for X-ray crystallographic analysis. Crystals were grown in a solution consisting of 0.1 M HEPES pH 8.0, 12%(w/v) polyethylene ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2011
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.06066-11